Today, Microsoft released version 1.2 of their Zune software, which includes support for Vista.
Operating system
Microsoft© Windows© Vista, Windows XP Home Edition Service Pack 2 (SP2), Windows XP Professional SP2, Windows XP Tablet PC Edition SP2, or Windows XP Media Center Edition 2005 with KB900325 (Rollup Update 2 for Windows XP Media Center Edition 2005).
However, still no support for Windows XP 64-bit edition, or Windows Server. But you weren't planning on using a Windows Server machine to store your music files at work, were you? I didn't think so.
Now, if Microsoft would just release PowerShell for Vista...